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Oxidant‐mediated proteolytic activation of Ca 2+ ‐ATPase in microsomes of pulmonary smooth muscle
Author(s) -
Ghosh Salil K.,
Chakraborti Tapati,
Michael John R.,
Chakraborti Sajal
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(96)00471-1
Subject(s) - microsome , aprotinin , chemistry , stimulation , atpase , protease , substrate (aquarium) , enzyme , biochemistry , medicine , endocrinology , biology , ecology
Treatment of bovine pulmonary artery smooth muscle tissue microsomes with H 2 O 2 (1 mM) markedly stimulated protease activity tested with a synthetic substrate p ‐nitroanilide (BAPNA), and also enhanced Ca 2+ ‐ATPase activity. ATP‐dependent Ca 2+ uptake was found to be stimulated upon treatment of the microsomes with H 2 O 2 . Pretreatment of the microsomes with vitamin E and aprotinin prevented the H 2 O 2 ‐induced stimulation of Ca 2+ ‐ATPase activity and also ATP‐dependent Ca 2+ uptake. In contrast, H 2 O 2 ‐induced inhibition of Na + ‐dependent Ca 2+ uptake was reversed by vitamin E and aprotinin.