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The cloning, expression and crystallisation of a thermostable arginase
Author(s) -
Bewley Maria C.,
Lott J.Shaun,
Baker Edward N.,
Patchett Mark L.
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(96)00459-0
Subject(s) - arginase , recombinant dna , cloning (programming) , microbiology and biotechnology , molecular cloning , thermophile , biochemistry , gene , escherichia coli , biology , t7 rna polymerase , enzyme , chemistry , gene expression , amino acid , arginine , bacteriophage , computer science , programming language
The gene for the thermostable arginase from the thermophilic bacterium ‘ Bacillus caldovelox ’ has been cloned and sequenced. Expression of recombinant arginase at high levels has been achieved in E. coli using an inducible T7 RNA polymerase‐based system. A facile purification procedure incorporating a heat‐treatment step yielded 0.2 g of recombinant arginase per litre of induced culture. The kinetic properties of the purified recombinant protein are essentially identical to the native enzyme. The recombinant protein has been crystallised and one crystal form is isomorphous to crystals of the native protein.