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Structures of new acidic O‐specific polysaccharides of the bacterium Proteus mirabilis serogroups O26 and O30
Author(s) -
Shashkov Alexander S.,
Toukach Filip V.,
Paramonov Nikolay A.,
Ziolkowski Andrzej,
Senchenkova Sof'ya N.,
Kaca Wieslaw,
Knirel Yuriy A.
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(96)00454-1
Subject(s) - proteus mirabilis , polysaccharide , bacteria , microbiology and biotechnology , chemistry , biology , biochemistry , genetics , staphylococcus aureus
The polysaccharide chains of the lipopolysaccharides of the Proteus mirabilis serogroups O26 and O30 were studied using sugar and methylation analysis and 1 H and 13 C NMR spectroscopy, including two‐dimensional correlation spectroscopy and rotating‐frame NOE spectroscopy. The polysaccharides were found to be acidic due to the presence of d ‐galacturonic acid and its amide with l ‐lysine in serogroup O26 or d ‐glucuronic acid in serogroup O30, and the structures of their tetrasaccharide repeating units were established. The O26‐specific polysaccharide is structurally and serologically related to the O‐specific polysaccharide of P. mirabilis O28, which includes amides of d ‐GalA with l ‐lysine and l ‐serine [Radziejewska‐Lebrecht, J. et al. (1995) Eur. J. Biochem. 230, 705–712].