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Calnexin : its molecular cloning and expression in the liver of the frog, Rana rugosa
Author(s) -
Yamamoto Satoshi,
Nakamura Masahisa
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(96)00443-7
Subject(s) - rana , cloning (programming) , biology , calnexin , zoology , microbiology and biotechnology , anatomy , calreticulin , endoplasmic reticulum , computer science , programming language
A 2.2‐kb cDNA clone encoding the endoplasmic reticulum (ER) resident protein, calnexin (CLX), was isolated from the frog Rana rugosa liver cDNA library and sequenced. The sequence encoded 622 amino acids and was 77% similar to mammalian CLXs and 56% to mouse CLX‐t. In the phylogenetic tree, mouse CLX‐t was clearly diverged from other CLXs including frog. The amino acid sequence of CLX showed regions similar to those in frog calreticulin (CLT), although CLX, but not CLT, contained a histidine‐rich region at the NH 2 ‐terminus. CLX gene expression was observed only in the liver among various tissues examined. Additionally, its expression was strong in the liver in 2‐month post‐metamorphosis frogs, but very weak in adults. The results suggest that the CLX gene is expressed in a tissue‐ and stage‐dependent manner in the frog, R. rugosa , and that CLX is widely distributed in eukaryotic organisms.