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Very low density lipoprotein receptor binds apolipoprotein E2/2 as well as apolipoprotein E3/3
Author(s) -
Takahashi Sadao,
Oida Koji,
Ookubo Minoru,
Suzuki Jinya,
Kohno Mitsuyuki,
Murase Toshio,
Yamamoto Tokuo,
Nakai Tsuguhiko
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(96)00439-5
Subject(s) - apolipoprotein c2 , low density lipoprotein receptor related protein 8 , apolipoprotein b , apolipoprotein e , chemistry , ldl receptor , lipoprotein , receptor , very low density lipoprotein , biochemistry , medicine , cholesterol , disease
The VLDL receptor, a newly identified lipoprotein receptor, recognizes apoE containing lipoproteins. The human VLDL receptor was overexpressed in ldlA‐7, a mutant Chinese hamster ovary cells lacking LDL receptors. Each VLDL obtained from a normolipidemic subject with two ε3 or ε2 alleles similarly competed for the binding of radiolabeled rabbit β‐VLDL to the VLDL receptors. The anti‐apoE monoclonal antibody 1D7, which inhibited binding of apoE3 to the LDL receptors, failed to compete for the binding of VLDL (apoE3 or apoE2) to the VLDL receptors. Results indicate that the binding site of apoE on the VLDL receptor may differ from its binding site on the LDL receptor.