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Possible nystatin‐protein interaction in yeast plasma membrane vesicles in the presence of ergosterol. A Förster energy transfer study
Author(s) -
Opekarová Miroslava,
Urbanová Petra,
Konopásek Ivo,
Kvasnička Peter,
Strzalka Kazimierz,
Sigler Karel,
Amler Evžen
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(96)00434-6
Subject(s) - nystatin , ergosterol , vesicle , membrane , chemistry , biophysics , yeast , membrane protein , biochemistry , biology , antibiotics
The Förster energy transfer from tryptophan residues of membrane proteins to nystatin was measured in reconstituted yeast plasma membrane vesicles free of, or doped with, ergosterol. We wanted to elucidate whether the functional change of membrane transport proteins from H + symporters to facilitators, observed in ergosterol‐containing plasma membrane vesicles on addition of nystatin [Opekarová and Tanner (1994) FEBS Lett. 350, 46–50], is reflected in altered protein‐nystatin relations within the membrane. Both frequency‐domain and time‐domain time‐resolved fluorescence spectroscopy showed that in the presence of ergosterol nystatin is located much closer to membrane proteins than in its absence.