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Molecular characterization of a new urea transporter in the human kidney
Author(s) -
Olivès Bernadette,
Martial Sonia,
Mattei Marie-Geneviève,
Matassi Giorgio,
Rousselet Germain,
Ripoche Pierre,
Cartron Jean-Pierre,
Bailly Pascal
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(96)00425-5
Subject(s) - complementary dna , microbiology and biotechnology , biology , urea , transporter , gene , xenopus , biochemistry , gene duplication , molecular cloning , homology (biology) , peptide sequence , cdna library , chemistry
A cDNA clone (HUT2) sharing 61.1% and 89.9% sequence identity with the human erythroid (HUT11) and the rabbit (UT2) urea transporters, respectively, was isolated by homology cloning from a human kidney library. HUT2 transcripts were restricted to the kidney and the HUT2 polypeptide was not immunoprecipitated with blood group Kidd‐related antibodies (anti‐Jk3) in coupled transcription‐translation assays. Functional expression studies in Xenopus oocytes demonstrated that HUT2‐mediated urea transport was not inhibited by p ‐chloromercuribenzene sulfonate (pCMBS) which, however, inhibited the urea flux mediated by HUT11. These findings demonstrate that at least two distinct urea transporters are present in human tissues. By in situ hybridization, the gene encoding HUT2 has been assigned to chromosome 18q12.1‐q21‐1, as found previously for the Kidd/urea transporter HUT11, suggesting that both genes evolved from duplication of a common ancestor.