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Intracellular free Ca 2+ dynamic changes to histamine are reduced in cystic fibrosis human tracheal gland cells
Author(s) -
Jacquot Jacky,
Maizières Michaël,
Spilmont Christophe,
Millot Jean-Marc,
Sébille Stéphane,
Merten Marc,
Kammouni Wafa,
Manfait Michel
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(96)00405-x
Subject(s) - histamine , exocytosis , cystic fibrosis , endocrinology , medicine , secretion , intracellular , chemistry , stimulation , biology , biochemistry
This study documents a difference between cystic fibrosis human (CF‐HTG) and normal human (HTG) tracheal gland cells: the ability of histamine to induce an increase of intracellular free calcium concentration [Ca 2+ ] i was abnormally reduced in CF‐HTG cells. The magnitude of the [Ca 2+ ] i peak rise in response to histamine is smaller in CF‐HTG cells than in HTG cells, and the percentage of CF‐HTG cells that increase [Ca 2+ ] i is decreased compared with HTG cells. In contrast to histamine, the human neutrophil elastase (HNE) stimulation of both CF‐HTG and HTG cells generated [Ca 2+ ] i asynchronous oscillations and the magnitude of the peak [Ca 2+ ] i response as well as the percentage of responding cells were similar for both groups. By videomicroscopy observations, the secretory response (exocytosis of secretion granules) of CF‐HTG cells occurred with HNE, but not with histamine, thus suggesting that [Ca 2+ ] i asynchronous oscillations may be linked to the exocytosis process in human tracheal gland cells.