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Flow cytometry analysis of α 1 ‐adrenoceptor subtypes
Author(s) -
Hirasawa Akira,
Tsumaya Keiko,
Awaji Takeo,
Shibata Katsushi,
Homma Nobuo,
Shinomiya Takahisa,
Tsujimoto Gozoh
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(96)00388-2
Subject(s) - prazosin , flow cytometry , bodipy , microbiology and biotechnology , adrenergic receptor , peptide , receptor , chemistry , chinese hamster ovary cell , antibody , cytometry , hamster , biology , fluorescence , biochemistry , antagonist , immunology , physics , quantum mechanics
To characterize the α 1 ‐adrenoceptor subtypes, we developed a flow cytometry method using the fluorescent ligand BODIPY‐FL prazosin and the anti‐peptide antibody against the α 1b ‐adrenoceptor amino terminus (designated 1B‐N1‐C) as probes. Three α 1 ‐adrenoceptors ( α 1a , β 1b and α 1d ) expresed in CHO cells were detected by BODIPY‐FL prazosin; however, only α 1b ‐adrenoceptor subtype was detected by the anti‐peptide antibody 1B‐N1‐C. Furthermore, the flow cytometry analysis with 1B‐N1‐C specifically identified α 1b ‐adrenoceptor in native cells of hamster DDT 1 ‐MF2 cells, rat hepatocytes and1 cardiomyocytes.