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Identification and immunohistochemical localization of macrophage migration inhibitory factor in human cornea
Author(s) -
Matsuda Akira,
Tagawa Yoshitsugu,
Matsuda Hidehiko,
Nishihira Jun
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(96)00386-9
Subject(s) - macrophage migration inhibitory factor , immunohistochemistry , cornea , polyclonal antibodies , biology , corneal epithelium , epithelium , microbiology and biotechnology , recombinant dna , antibody , immunology , gene , cytokine , biochemistry , genetics , neuroscience
We identified macrophage migration inhibitory factor (MIF) mRNA expression in human cornea, and demonstrated its immunohistological localization. Reverse transcription‐polymerase chain reaction analysis revealed that MIF mRNA was expressed in both the corneal epithelial and endothelial cells. Immunohistochemical study using the polyclonal antibody prepared from immunizing a rabbit with human recombinant MIF showed that MIF was present in the basal cells of corneal epithelium and endothelial cells. The fact that MIF exists in those cells of the cornea indicates that MIF may play an important role in corneal cell immunity and cellular differentiation.