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Modified 2S albumins with improved tryptophan content are correctly expressed in transgenic tobacco plants
Author(s) -
Marcellino Lucilia H.,
Neshich Goran,
Grossi de Sá Maria F.,
Krebbers Enno,
Gander Eugen S.
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(96)00375-4
Subject(s) - tryptophan , nicotiana tabacum , transgene , gene , genetically modified crops , mutant , biology , biochemistry , amino acid , genetics
Brazil nut 2S albumins lack the essential amino acid tryptophan. In order to improve the protein's nutritional value and create a basis for structural investigations, three separate modified Brazil nut 2S albumin genes were constructed. The first mutant contains five consecutive tryptophan codons, while the other two modified genes encode proteins carrying single tryptophan residues at sites that will allow confirmation of the predicted protein structure through fluorescence quenching techniques. The modified genes, under the regulation of the CaMV 35S promoter, were introduced into Nicotiana tabacum . All three modified genes were correctly transcribed and the 2S albumin accumulated in the seeds of transgenic plants.