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Initial analysis of 750 MHz NMR spectra of selectively 15 N‐G,U labelled E. coli 5S rRNA
Author(s) -
Grüne Matthias,
Görlach Matthias,
Soskic Vukic,
Klussmann Sven,
Bald Rolf,
Fürste Jens Peter,
Erdmann Volker A.,
Brown Larry R.
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(96)00361-4
Subject(s) - heteronuclear molecule , rna , guanine , ribosomal rna , molecule , nucleotide , uridine , chemistry , crystallography , base pair , nuclear magnetic resonance spectroscopy , stereochemistry , biochemistry , dna , organic chemistry , gene
The overall folding of an RNA molecule is reflected in its base pairing pattern. The identification of that pattern provides a first step towards the determination of the structure of an RNA molecule. We show that the application of heteronuclear NMR methods at 750 MHz to E. coli 5S rRNA (120 nucleotides) selectively labelled with 15 N in guanine and uridine allows observation of base pairing patterns for a larger RNA molecule. We also present evidence that the fold of the E‐domain of the 5S rRNA (nt 79–97) as a contiguous part of the 5S rRNA and as an isolated molecule is virtually the same.