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Expression of GCAP 1 and GCAP2 in the retinal degeneration ( rd ) mutant chicken retina
Author(s) -
Semple-Rowland Susan L.,
Gorczyca Wojciech A.,
Buczylko Janina,
Helekar Bharati S.,
Ruiz Claudia C.,
Subbaraya Iswari,
Palczewski Krzysztof,
Baehr Wolfgang
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(96)00345-6
Subject(s) - retina , visual phototransduction , biology , mutant , retinal degeneration , retinal , downregulation and upregulation , microbiology and biotechnology , anatomy , genetics , gene , biochemistry , neuroscience
We cloned the guanylate cyclase activating proteins, GCAP1 and GCAP2, from chicken retina and examined their expression in normal and predegenerate rd/rd chicken retina. Northern analyses show that the amounts of the single transcripts encoding GCAPI and GCAP2 are reduced to about 70% of normal levels in rdlrd retina. Western analyses reveal that GCAP2 levels appear normal in this retina, while GCAPI levels are reduced by more than 90%. The specific downregulation of GCAPI in rd/rd retina is consistent with a model for this disease in which activation of guanylate cyclase in the photoreceptors is abnormal, resulting in low levels of cGMP and an absence of phototransduction.