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Molecular cloning and characterization of a novel orphan receptor (P 2P ) expressed in human pancreas that shows high structural homology to the P 2U purinoceptor
Author(s) -
Stam Nico J.,
Klomp Jan,
van de Heuvel Marcel,
Olijve Wiebe
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(96)00321-3
Subject(s) - gene , purinergic receptor , biology , homology (biology) , cloning (programming) , genetics , receptor , molecular cloning , microbiology and biotechnology , peptide sequence , computer science , programming language
Here we report the cloning of a gene encoding a new member of the superfamily of G protein‐coupled receptors. The gene encodes a protein of 365 amino acids closely resembling two recently cloned nucleotide binding receptors, called P 2U and P 2Y purinoceptors (71% and 49% sequence identity within the transmembrane domains, respectively). Our studies show that this new putative purinoceptor (designated P 2P ) is encoded by an intronless single copy gene that is exclusively expressed in pancreas, in contrast to the P 2U and the P 2Y purinoceptors which are widely distributed throughout the periphery. The identification of a pancreas‐specific human putative P 2 purinoceptor makes it attractive to speculate that the reported actions of ADP/ATP analogues in pancreas on insulin secretion are mediated through this receptor.