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The structural origin of the color differences in the bioluminescence of firefly luciferase
Author(s) -
Yoshihiro Ohmiya,
Takashi Hirano,
Mamoru Ohashi
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(96)00288-8
Subject(s) - bioluminescence , luciferase , firefly protocol , luciferases , chemistry , luminescent measurements , biochemistry , luciferin , biophysics , biology , zoology , luminescence , physics , optics , transfection , gene
Six chimeric mutants between Hotaria parvula ( λ max=568 nm) and Pyrocoelia miyako ( λ max=550 nm) luciferases were reconstructed to determine the structural origin of the color differences in firefly luciferase. Based on light‐emitting color, five chimeric luciferases could be divided into two groups: the three green‐emitting mutants, classified as P. miyako luciferase, and the two yellow‐emitting mutants, classified as H. parvula luciferase. Their common fragments between Val‐209 and Ala‐318 within each group contain the active site for the color differences.