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DNA affinity to biological membranes is enhanced due to complexation with hydrophobized polycation
Author(s) -
Yaroslavov A.A.,
Sukhishvili S.A.,
Obolsky O.L.,
Yaroslavova E.G.,
Kabanov A.V.,
Kabanov V.A.
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(96)00281-5
Subject(s) - chemistry , liposome , membrane , bromide , dna , phosphatidylcholine , cationic polymerization , chromatography , organic chemistry , biochemistry , phospholipid
The interaction of negatively charged liquid phosphatidylcholine/cardiolipin liposomes with water‐soluble negatively charged DNA/cetylpyridinium bromide and DNA/poly( N ‐alkyl‐4‐vinylpyridinium bromide) complexes was studied. It is shown that the DNA/cetylpyridinium bromide complex while interacting with the liposomes is destroyed, so that the cetylpyridinium cation is incorporated into the liposomal membrane and DNA remains in the solution. The DNA/poly‐( N ‐ethyl‐4‐vinylpyridinium bromide) complex does not interact at all with the liposomes. On the contrary, the complex of DNA with the poly(vinylpyridinium) cation carrying a small amount of N ‐cetyl groups is adsorbed on the membrane as a whole. The data obtained indicate that complexation of DNA with hydrophobized polycations can be used for enhancing DNA affinity to biological membranes.