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Sodium channel mRNA in the B104 neuroblastoma cell line
Author(s) -
Dib-Hajj S.D.,
Hinson A.W.,
Black J.A.,
Waxman S.G.
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(96)00273-6
Subject(s) - neuroblastoma , messenger rna , chemistry , sodium , cell culture , sodium channel , biology , biochemistry , gene , genetics , organic chemistry
B104 neuroblastoma cells are excitable, but the ion channels underlying electrogenesis in these cells have not been identified. RT‐PCR, restriction enzyme analysis and in situ hybridization were used to study sodium channel mRNAs in B104 cells. High levels of sodium channel α‐subunit mRNAs III, NaG and Na6 and β1‐subunit mRNA were detected by RT‐PCR in B104 cells. Low levels of types I and II α‐subunit mRNAs were also present. In situ hybridization with subtype‐specific riboprobes detected sodium channel α‐subunit mRNAs III, NaG and Na6 and β1‐subunit mRNA in B104 cells; analysis of the percentage of B104 cells expressing each α‐subunit mRNA subtype suggests that some cells express the mRNAs for several α‐subunits.