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Degradation of glutathione S ‐conjugates by a carboxypeptidase in the plant vacuole
Author(s) -
Wolf Andreas E.,
Dietz K.-J.,
Schröder P.
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(96)00272-4
Subject(s) - vacuole , glutathione , conjugate , xenobiotic , biochemistry , chemistry , cytosol , carboxypeptidase , in vitro , enzyme , cytoplasm , mathematical analysis , mathematics
For plants, glutathione conjugation is a major pathway to detoxify organic xenobiotic. Glutathione S ‐conjugates (SG‐conjugates) are formed in the cytosol, the in vitro transport over the tonoplast has been described and a final storage in the vacuole has been postulated. We show here that alachlor rapidly accumulates as GS‐conjugates in the plant vacuole and that the first step of its degradation, the formation of the respective γ ‐glutamylcysteinyl‐ S ‐conjugate, is catalyzed by a vacuolar carboxypeptidase. These results suggest the glutathione conjugate as a transport form but not a storage form of xenobiotic molecules.