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Hydrogen peroxide and sequence‐specific DNA damage in human cells
Author(s) -
Burdon R.H.,
Gill Vera,
Boyd Patricia A.,
Rahim Raha Abdul
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(96)00230-x
Subject(s) - hydrogen peroxide , sequence (biology) , dna damage , dna , chemistry , microbiology and biotechnology , computational biology , biochemistry , biology
Exposure of HeLa cells in monolayer culture to increasing concentrations of exogenously added H 2 O 2 causes damage to cellular DNA. When the DNA is subsequently isolated from the non‐apoptotic cells remaining in such cultures, evidence was obtained to suggest that the DNA damage elicited in intact cells was non‐random and that certain nucleotide sequences associated with, or related to, the genes for heat shock protein 60 and catalase were more susceptible to damage than others. In contrast, these particular sequences were not specifically susceptible to damage when naked human DNA was exposed directly to H 2 O 2 in vitro. On an overall comparative basis, sequences in the genes encoding catalase, α‐1 antitrypsin and β‐actin appear more vulnerable to H 2 O 2 in vivo, than sequences in H‐ras and the p53 gene which seem surprisingly resistant.