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Acidic phospholipids inhibit the phospholipase D activity of rat brain neuronal nuclei
Author(s) -
Kanfer J.N.,
McCartney D.G.,
Singh I.N.,
Freysz L.
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(96)00205-0
Subject(s) - phosphatidic acid , phospholipase d , phospholipase , phospholipase a2 , hydrolysis , chemistry , phospholipase a1 , biochemistry , phospholipase c , disinhibition , phospholipase a , enzyme , phospholipid , biology , neuroscience , membrane
An oleate dependent form of phospholipase D is present in rat brain neuronal nuclei and both the hydrolytic and transphosphatidylation activities measured. Several acidic phospholipids were found to inhibit this activity in a dose dependent manner. The IC 50 values varied from 3.5 μM for PIP 2 to 200 μM for phosphatidic acid. The hydrolysis of PIP 2 by phospholipase C would be expected to result in the disinhibition of the oleate dependent phospholipase D activity.