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Vigilin contains a functional nuclear localisation sequence and is present in both the cytoplasm and the nucleus
Author(s) -
Kügler S.,
Grünweller A.,
Probst C.,
Klinger M.,
Müller P.K.,
Kruse C.
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(96)00204-9
Subject(s) - cytoplasm , nucleus , rna , cloning (programming) , nuclear localization sequence , biology , microbiology and biotechnology , nls , genetics , gene , computer science , programming language
Vigilin is a member of the KH protein family and contains 14 tandemly arranged potential RNA‐binding domains. Between KH domains 2 and 3 we have identified a nuclear localization sequence by cloning this sequence into the NH 2 ‐terminal region of phage T7 RNA polymerase as a reporter protein and by showing its transfer into the nucleus. Furthermore we provide experimental evidence that Vigilin is present both in the nucleus and in the cytoplasm in similar concentrations. These observations support the notion that Vigilin may shuttle between nucleus and cytoplasm presumably in contact with RNA molecules.