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T7 DNA‐dependent RNA polymerase can transcribe RNA from tick‐borne encephalitis virus (TBEV) cDNA with SP6 promoter
Author(s) -
Dobrikova E.Yu.,
Pletnev A.G.,
Karamyshev V.N.,
Morozova O.V.
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(96)00201-3
Subject(s) - t7 rna polymerase , rna dependent rna polymerase , polymerase , rna polymerase i , microbiology and biotechnology , rna , biology , transcription (linguistics) , rna polymerase , rna polymerase ii , small nuclear rna , virology , dna , promoter , gene expression , gene , genetics , bacteriophage , linguistics , philosophy , escherichia coli
T7 RNA polymerase is shown to recognize the SP6 promoter including 17 base pairs before the transcription start site and produce the 5′‐end TBEV RNA. The yield of TBEV RNA synthesized by heterologous T7 RNA polymerase from cDNA construction with SP6 promoter is higher than the RNA production by homologous SP6 RNA polymerase. The addition of 1 pmol template DNA with SP6 17 bp promoter in transcription mixture for SP6 or T7 RNA polymerases resulted in 1–5 × 10 −2 pmol RNA production.