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Apoptosis is triggered by the cyclic AMP signalling pathway in renal mesangial cells
Author(s) -
Mühl Heiko,
Nitsch Dorothea,
Sandau Katrin,
Brüne Bernhard,
Varga Zoltan,
Pfeilschifter Josef
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(96)00179-2
Subject(s) - apoptosis , dna fragmentation , microbiology and biotechnology , mesangial cell , forskolin , chemistry , signal transduction , medicine , endocrinology , receptor , biology , programmed cell death , biochemistry , kidney
Glomerular mesangial cells are regarded as specialized smooth muscle cells located within the renal glomeruli and fulfilling important functions in glomerular physiology and pathophysiology. Here, we report that activation of the cyclic AMP signalling pathway by dibutyryl cyclic AMP, forskolin, or the β 2 ‐adrenergic receptor agonist salbutamol results in induction of apoptosis in mesangial cells. Activation of the apoptotic programme results in DNA fragmentation which is visible for most forms of apoptosis and is paralleled by enrichment of cytosolic DNA/histone complexes, an increasing number of cellular 3′‐OH‐fragmented DNA ends and typical nuclear chromatin condensation. Induction of apoptosis was found to be dependent on translation and independent of nitric oxide synthase activity.