Premium
Immunolocalization of X‐arrestin in human cone photoreceptors
Author(s) -
Sakuma H.,
Inana G.,
Murakami A.,
Higashide T.,
McLaren M.J.
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(96)00163-9
Subject(s) - arrestin , cone (formal languages) , microbiology and biotechnology , chemistry , biophysics , neuroscience , biology , computer science , signal transduction , g protein coupled receptor , algorithm
X‐arrestin is a recently identified retina‐specific gene of unknown function. Affinity‐purified anti‐peptide antibody to human X‐arrestin was prepared, and used in Western blot analysis of human retinal proteins and for immunohistochemistry on human retinal sections. By Western blot analysis, the antibody specifically bound to an ≈47 kDa protein, and by indirect immunofluorescence specifically labeled cone photoreceptors with greatest intensity in their outer segments. In single and double label experiments, the localization of X‐arrestin immunoreactivity was compared with immunolabelling patterns obtained with antibodies to red/green cone opsin, rhodopsin, and S‐antigen. The results showed that X‐arrestin is expressed in red‐, green‐ and blue‐sensitive cones in the human retina.