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cDNA cloning and characterization of A3i, an alternatively spliced rat A3 adenosine receptor variant
Author(s) -
Sajjadi Fereydoun G.,
Boyle David L.,
Domingo Ron C.,
Firestein Gary S.
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(96)00150-0
Subject(s) - complementary dna , microbiology and biotechnology , biology , 5 ht5a receptor , alternative splicing , rna splicing , intron , receptor , rapid amplification of cdna ends , messenger rna , molecular cloning , rna , gene , biochemistry
A cDNA encoding a variant form of the A3 adenosine (Ado) receptor was isolated from rat by reverse transcription of brain mRNA followed by PCR. The full‐length receptor (A3i) cDNA encodes 337 amino acids and shares complete sequence identity with the rat A3 Ado receptor, except for the presence of a seventeen amino acid insert located in the second intracellular domain. In contrast to the rat A3 receptor, stable expression of A3i in CHO cells resulted in poor coupling to Gl proteins Analysis of receptor transcripts by RT‐PCR suggests that the A3 Ado receptor mRNAs are products of alternative splicing. Sequence analysis of A3 genomic DNA Identified a 1.7 kb intron that is likely alternatively spliced to produce the A3 and A3i receptors.