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A link between ferritin gene expression and ribonucleotide reductase R2 protein, as demonstrated by retroviral vector mediated stable expression
Author(s) -
Fan Huizhou,
Villegas Cristy,
Wright Jim A.
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(96)00143-3
Subject(s) - ribonucleotide reductase , microbiology and biotechnology , gene expression , ferritin , expression vector , recombinant dna , complementary dna , ribonucleotide , biology , vector (molecular biology) , gene , chemistry , biochemistry , nucleotide , protein subunit
We have constructed a retroviral expression vector for the mammalian ribonucleotide reductase R2 component. Stable infectants, which express a myc epitope tagged R2 protein from the vector cDNA were obtained and described for the first time. Cells containing the recombinant protein exhibited increased ribonucleotide reductase activity, and were resistant to the antitumour agent hydroxyurea, which targets the R2 component of ribonucleotide reductase. Furthermore, a direct link between ferritin gene expression and R2 protein was observed, since cells containing vector expressed recombinant R2 protein exhibited increased H‐chain and L‐chain ferritin gene expression.