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Rabbit translation elongation factor 1α stimulates the activity of homologous aminoacyl‐tRNA synthetase
Author(s) -
Negrutskii Boris S,
Budkevich Tatyana V,
Shalak Vyacheslav F,
Turkovskaya Galina V,
El'Skaya Anna V
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(96)00128-7
Subject(s) - aminoacyl trna , transfer rna , eukaryotic translation elongation factor 1 alpha 1 , protein biosynthesis , translation (biology) , elongation factor , ef tu , aminoacyl trna synthetase , homologous chromosome , biochemistry , initiation factor , eif2 , biology , eukaryotic translation , chemistry , microbiology and biotechnology , rna , ribosome , messenger rna , gene
Functional and structural sequestration of aminoacyl‐tRNA has been recently found in eukaryotic cells and the aminoacyl‐tRNA channeling has been suggested [B.S. Negrutskii et al., Proc. Natl. Acad. Sci. 91 (1994) 964–968], but molecular details and mechanism of the process remained unclear. In this paper we have verified a possible interaction between rabbit aminoacyl‐tRNA synthetase and homologous translation elongation factor 1α (EF‐1α), the proteins which may play a role of sequential components involved into the transfer of the aminoacyl‐tRNA along the protein synthetic metabolic chain. The stimulation of the phenylalanyl‐tRNA synthetase activity by EF‐1α is found. The effect is shown to be specific towards the origin of tRNA and elongation factor molecules. The data obtained favor the direct transfer mechanism of the aminoacyl‐tRNA channeling process during eukaryotic protein synthesis.