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Solubility of artificial proteins with random sequences
Author(s) -
Prijambada Irfan D,
Yomo Tetsuya,
Tanaka Fumihiro,
Kawama Toshihiro,
Yamamoto Keizo,
Hasegawa Akihisa,
Shima Yasufumi,
Negoro Seiji,
Urabe Itaru
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(96)00123-8
Subject(s) - solubility , chemistry , biophysics , computational biology , biology , organic chemistry
A library of artificial random proteins of 141 amino acid residues of which 95 are random and which includes the 20 kinds of amino acids was prepared. Out of the 25 identified random proteins, 5 were soluble in the cell lysate, indicating that about 20% of the random proteins expressed in Escherichia coli are expected to be soluble. The soluble random proteins RP3–42 and RP3–45 and insoluble RP3–70 were purified. The solubility of the purified form is the same as that in the cell lysate.