Premium
Identification of macrophage migration inhibitory factor (MIF) in human skin and its immunohistochemical localization
Author(s) -
Shimizu Tadamichi,
Ohkawara Akira,
Nishihira Jun,
Sakamoto Wataru
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(96)00120-2
Subject(s) - macrophage migration inhibitory factor , epidermis (zoology) , immunohistochemistry , polyclonal antibodies , human skin , western blot , recombinant dna , biology , microbiology and biotechnology , blot , antibody , basal (medicine) , inflammation , immunology , cytokine , endocrinology , gene , biochemistry , genetics , anatomy , insulin
The presence and tissue localization of macrophage migration inhibitory factor (MIF) in human skin were examined. Reverse transcription‐polymerase chain reaction analysis revealed that MIF mRNA was expressed in both surgically obtained normal human epidermis and primary cultured human keratinocytes. The expression of MIF was further confirmed by Western blot analysis, which demonstrated a single band at about 12.5 kDa using a polyclonal antibody against human recombinant MIF. Immunohistochemical studies showed that MIF existed in human epidermis, especially in the basal layer. The pathophysiological role of MIF in human skin remains undefined; however, the present results indicate that MIF may play an important role in immunity, inflammation and cellular differentiation of epidermal cells.