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In vitro import of pre‐ferredoxin‐NADP + ‐oxidoreductase from Cyanophora paradoxa into cyanelles and into pea chloroplasts
Author(s) -
Jakowitsch Johannes,
Neumann-Spallart Christoph,
Ma Yan,
Steiner Jürgen,
Schenk Hainfried E.A.,
Bohnert Hans J.,
Löffelhardt Wolfgang
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(96)00102-0
Subject(s) - chloroplast , plastid , ferredoxin , biology , organelle , heterologous , oxidoreductase , genome , botany , biochemistry , gene , enzyme
Using a novel cyanelle isolation procedure we showed that pre‐ferredoxin‐NADP + ‐oxidoreductase (pre‐FNR) from C. paradoxa is translocated in vitro across the peptidoglycancontaining cyanelle envelope. Efficient import was also observed in a heterologous system with pea chloroplasts as the recipient organelles. These results support the conclusion derived from comparative analysis of plastid genome organization, that all plastids originate from a common semi‐autonomous endosymbiotic ancestor.