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Transformation of Saccharomyces cerevisiae with a cDNA encoding a sterol C ‐methyltransferase from Arabidopsis thaliana results in the synthesis of 24‐ethyl sterols
Author(s) -
Husselstein Tania,
Gachotte Daniel,
Desprez Thierry,
Bard Martin,
Benveniste Pierre
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(96)00089-0
Subject(s) - arabidopsis thaliana , saccharomyces cerevisiae , sterol , transformation (genetics) , complementary dna , methyltransferase , arabidopsis , biology , genetics , biochemistry , chemistry , yeast , gene , mutant , methylation , cholesterol
Using an EST‐cDNA probe, a full‐length cDNA (411) sequence of 1411 bp was isolated from A. thaliana . This sequence contained features typical of methyltransferases in general and in particular showed 38% identity with ERG6 , a S. cerevisiae gene which encodes the zymosterol‐C‐24‐methyltransferase. A yeast vector containing this ORF (4118‐pYeDP60) was used to transform a wild type S. cerevisiae which accumulates predominantly ergosterol, a 24‐methyl sterol as well as a mutant erg6 null mutant accumulating principally zymosterol, a sterol non‐alkylated at C‐24. In both cases, several 24‐ethyl‐ and 24‐ethylidene sterols were synthetized indicating that the 4118 cDNA encodes a plant sterol C ‐methyltransferase able to perform two sequential methylations of the sterol side chain.