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Functional characterisation of the Drosophila 5‐HT dro 1 and 5‐HT dro2B serotonin receptors in insect cells: activation of a G α s ‐like protein by 5‐HT dro 1 but lack of coupling to inhibitory G‐proteins by 5‐HT dro2B
Author(s) -
Obosi Louis A.,
Schuette Diana G.,
Europe-Finner G.Nicholas,
Beadle David J.,
Hen René,
King Linda A.,
Bermudez Isabel
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(96)00078-6
Subject(s) - adenylyl cyclase , g protein , gs alpha subunit , pertussis toxin , receptor , 5 ht receptor , g protein coupled receptor , serotonin , biology , microbiology and biotechnology , alpha (finance) , adcy9 , biochemistry , medicine , construct validity , nursing , patient satisfaction
Insect cells are routinely used for the production of receptor proteins. Expression of the Drosophila 5‐HT dro 1 serotonin receptor resulted in positive coupling of the receptor to adenylyl cyclase via the G α s G‐protein subtype. The Drosophila 5‐HT dro2B receptor stimulated the metabolism of inositol phospholipid via a pertussis toxin‐insensitive G‐protein, but exhibited no detectable inhibition of adenylyl cyclase. Immunoblot analysis of the endogenous G‐proteins revealed that Sf9 cells lack the G‐protein subtypes G α i 1–3 and G α0 , but express the subtypes G α s and G α q .