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NF‐κB p50 subunit cross‐linking to DNA duplexes, containing a monosubstituted pyrophosphate internucleotide bond
Author(s) -
Kubareva Elena A.,
Fedorova Olga A.,
Gottikh Marina B.,
Tanaka Hiroaki,
Malvy Claude,
Shabarova Zoe A.
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(96)00072-5
Subject(s) - chemistry , dna , protein subunit , pyrophosphate , stereochemistry , duplex (building) , p50 , biochemistry , transcription factor , gene , enzyme
The new express technique based on the use of BrCN to synthesize DNA duplexes, containing non‐substituted or monosubstituted pyrophosphate internucleotide bonds has been proposed. Using this technique, DNA duplexes having modified internucleotide bonds between dT and dC residues in the human NF‐κB transcription factor recognition sequence in HIV‐1 (5′‐GGAAAGTCCC‐3′) have been prepared. We demonstrate that these internucleotide bonds within the recognition site do not prevent the formation of NF‐κB p50 subunit complex with the corresponding duplexes. The cross‐linking of NF‐κB p50 subunit to the DNA duplex containing a monosubstituted pyrophosphate internucleotide bond has been successfully performed.