Premium
A functional Spo0A is required for maximal aprE expression in Bacillus subtilis
Author(s) -
Olmos Jorge,
Bolaños Victor,
Causey Stuart,
Ferrari Eugenio,
Bollvar Francisco,
Valle Fernando
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(96)00070-1
Subject(s) - bacillus subtilis , operon , mutant , lac operon , mutation , genetics , biology , microbiology and biotechnology , gene expression , gene , bacteria
The initiation of sporulation in Bacillus subtilis is under control of the transcriptional factor SpoOA. Most SpoOA mutants fail to initiate the sporulation process and all the sporulation initiated processes such as the synthesis of subtilisin. However, the product of spoOA9V , one of the several spoOA mutants characterized, distinguishes itself in the fact that, while it appears to effectively repress abrB, it fails to activate the spoIIA operon. The aim of this study was to examine the effect of the spoOA9V mutation on aprE expression and we found that in different genetic backgrounds, the spoOA9V mutation has a negative effect on aprE::lacZ expression.