Premium
Isolation and properties of PS II membrane fragments depleted of the non heme iron center
Author(s) -
Kurreck J.,
Garbers A.,
Reifarth F.,
Andréasson L.-E.,
Parak F.,
Renger G.
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(96)00061-0
Subject(s) - chemistry , heme , population , photochemistry , cytochrome , hemeprotein , spectroscopy , biophysics , biochemistry , biology , enzyme , physics , demography , quantum mechanics , sociology
The functional properties and the content of non heme iron and cytochrome b 559 were investigated by measuring flash induced transient changes of the relative fluorescence quantum yield and applying Mössbauer spectroscopy. It was found that untreated PS II membrane fragments contain a heterogeneous population of two types of non heme iron centers and about 2 cytochrome b 559 per PS II. Twofold treatment of these samples with a recently described ‘iron depletion’ procedure (MacMillan, F., Lendzian, F., Renger, G. and Lubitz, W. (1995) Biochemistry 34, 3144–8156) leads to a complete loss (below the detection limit of Mössbauer spectroscopy) of the non heme iron center while more than 50% of the PS II complexes retain the functional integrity for light induced formation of the ‘stable’ radical pair Y Z OX P680Pheo Q A −. . This sample type deprived of virtually all non heme iron in PS II provides a most suitable material for magnetic resonance studies that require an elimination of the interaction between Fe 2+ and nearby radicals.