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A specific G o heterotrimer couples somatostatin receptors to voltage‐gated calcium channels in RINm5F cells
Author(s) -
Degtiar Vadim E.,
Wittig Burghardt,
Schultz Günter,
Kalkbrenner Frank
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(96)00047-6
Subject(s) - somatostatin , somatostatin receptor , voltage dependent calcium channel , g protein , receptor , somatostatin receptor 1 , somatostatin receptor 3 , agonist , chemistry , calcium , endocrinology , delta cell , medicine , microbiology and biotechnology , biology , biochemistry , somatostatin receptor 2 , insulin , glucagon
The peptide hormone somatostatin inhibits glucose‐induced insulin secretion in the rat insulinoma RINm5F cells by inhibition of voltage‐gated calcium channels. Here we used microinjection of antisene oligonucleotides directed against subtypes of G‐protein subunits to determine the subunit composition involved in somatostatin‐induced inhibition of voltage‐gated calcium channels in RINmF5 cells. Injection of antisene oligonucleotides annealing to the respective mRNA of Gα o2 , Gβ 1 and Gγ 3 reduced the somatostatin‐induced inhibition of calcium channels in these cells. Injection of antisene oligonucleotides directed against other G‐protein subunits did not, suggesting that in RINm5F cells the somatostatin receptor couples to a G protein of Gα o2 β 1 γ 3 composition. By using a selective agonist of type 2 somatostatin receptors (SSTR 2) NC 8–12, we identified this receptor as the subtype coupling to calcium channels in RINm5F cells.