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SGP28, a novel matrix glycoprotein in specific granules of human neutrophils with similarity to a human testis‐specific gene product and to a rodent sperm‐coating glycoprotein
Author(s) -
Kjeldsen Lars,
Cowland Jack B.,
Johnsen Anders H.,
Borregaard Niels
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(96)00030-0
Subject(s) - complementary dna , glycoprotein , microbiology and biotechnology , biology , gene , cdna library , biochemistry , peptide sequence , gene product , gene expression
A novel 28 kDa glycoprotein was purified from exocytosed material from human neutrophils and its primary structure partially determined. Degenerate oligonucleotide primers were used to amplify cDNA clones from a human bone marrow cDNA library. The deduced 245 amino acid sequence of the 2124 bp full‐length cDNA showed high degrees of similarity to the deduced sequences of the human gene TPX‐1 and of sperm coating glycoprotein from rat and mouse. Subcellular fractionation of human neutrophils indicated that the protein is localized in specific granules. The protein was named SGP28 (specific granule protein of 28 kDa).