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Ionic transport in the plasma membrane of carrot protoplasts from embryogenic cell‐suspension cultures
Author(s) -
Bregante Monica,
Gambale Franco,
LoSchiavo Fiorella
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(96)00016-6
Subject(s) - protoplast , biophysics , ionic bonding , patch clamp , ion channel , kinetics , ion , suspension (topology) , ion transporter , membrane , chemistry , potassium channel , membrane potential , analytical chemistry (journal) , chromatography , biology , biochemistry , physics , receptor , mathematics , organic chemistry , quantum mechanics , homotopy , pure mathematics
Ionic transport properties of protoplasts obtained from embryogenic carrot suspension cells were studied by the patch‐clamp technique. In the whole‐cell configuration, carrot protoplasts presented macroscopic time‐dependent outward currents, showing kinetics of activation which did not depend appreciably on the amplitude of the stimulus. Time‐ and voltage‐dependent whole‐cell inward rectifying currents as well as instantaneous non‐selective currents were also observed. Both time‐dependent inward and outward currents are carried by potassium ions. In a cell‐attached configuration, two types of single‐channel signals, displaying conductances of 10 and 17 pS, were observed; the instantaneous 10 pS channel was also present in outside‐out excised patches.