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Partial purification and characterization of a protein kinase that is activated by nuclear localization signal peptides
Author(s) -
Kurihara Toshinao,
Hori Masatsugu,
Takeda Hiroshi,
Inoue Michitoshi,
Yoneda Yoshihiro
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(96)00010-5
Subject(s) - nls , nuclear localization sequence , cytoplasm , phosphorylation , cytosol , biology , in vitro , protein kinase a , microbiology and biotechnology , biochemistry , kinase , xenopus , immunoprecipitation , enzyme , gene
A nuclear localization signal (NLS) is required for the transport of karyophilic proteins from the cytoplasm to the nucleus. In this study, NLS was examined in terms of its effect on diverse cellular functions such as protein phosphorylation reactions. When synthetic peptides containing the NLS of SV40 T‐antigen were injected into the cytoplasm of Xenopus oocytes, and the oocytes incubated with [ 32 P]phosphorus‐containing medium, a 32 kDa protein was found to be preferentially phosphorylated in an NLS‐dependent manner. The incubation of fractionated cytosolic extracts prepared from mouse Ehrlich ascites tumor cells with [ γ ‐ 32 P]ATP in the presence of the NLS peptides, results in the stimulation of the phosphorylation of several proteins. Similar in vitro stimulation was observed by other functional NLS peptides such as those of polyoma virus T‐antigen and nucleoplasmin. Little or no stimulation, however, was detected for peptides of mutant type and reverse type NLS of SV40 T‐antigen, and the C‐terminal portion of lamin B. Using an in vitro assay, the phosphorylation activity was fractionated chromatographically and a fraction was obtained which contained a high level of activity. The fraction was found to contain three major proteins having molecular masses of 95, 70, and 43 kDa. The in vivo and in vitro results are consistent with the existence of a protein kinase, called NLS kinase, that is specifically activated by NLS peptides.