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Inhibition of the skeletal muscle ryanodine receptor calcium release channel by nitric oxide
Author(s) -
Mészáros LászlóG.,
Minarovic Igor,
Zahradnikova Alexandra
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(96)00003-8
Subject(s) - ryanodine receptor , nitric oxide , skeletal muscle , chemistry , calcium , biophysics , calcium channel , medicine , endocrinology , biology , organic chemistry
NO donors were found to reduce the rate of Ca 2+ release from isolated skeletal muscle sarcoplasmic reticulum (SR) and the open probability of single ryanodine receptor Ca 2+ release channels (RyRCs) in planar lipid bilayers, and these effects were prevented by the NO quencher hemoglobin and reversed by 2‐mercaptoethanol. Ca 2+ release assessed in skeletal muscle homogenates was also reduced by NO that was generated in situ from l ‐arginine by endogenous, nitro‐ l ‐arginine methylester‐sensitive NO‐synthase. The effect of NO on the RyRC might explain NO‐induced depression of contractile force in striated muscles and, since both RyRC isoforms and NOS isoenzymes are ubiquitous, may represent a wide‐spread feedback mechanism in Ca 2+ signaling; i.e. Ca‐dependent activation of NO production and NO‐evoked reduction of Ca 2+ release from intracellular Ca 2+ stores.