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Expression of cytochrome P ‐450 3A in HT29‐MTX cells and caco‐2 clone TC7
Author(s) -
Carrière V,
Lesuffleur T,
Barbat A,
Rousset M,
Dussaulx E,
Costet P,
de Waziers I,
Beaune P,
Zweibaum A
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)91031-x
Subject(s) - microbiology and biotechnology , clone (java method) , biology , chemistry , gene , biochemistry
HT-29 sublines and Caco-2 clones were analyzed for the expression of cytochrome P-450 3A. The enzyme was found to be expressed in differentiated HT-29 cells selected by resistance to methotrexate and in one of seven Caco-2 clones, TC7. Its expression parallels the differentiation process, with highest levels being observed at late contluency. P-450 3A mRNA and protein patterns, as well as subcellular distribution, are intermediate between those observed in human adult intestine and fetal liver.