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The H + /ATP coupling ratio of the ATP synthase from thiol‐modulated chloroplasts and two cyanobacterial strains is four
Author(s) -
Van Walraven Hendrika S.,
Strotmann Heinrich,
Schwarz Oliver,
Rumberg Bernd
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)01536-1
Subject(s) - atp synthase , chemiosmosis , atp hydrolysis , chloroplast , proton , thiol , enzyme , electrochemical gradient , chemistry , coupling (piping) , atp synthase gamma subunit , hydrolysis , biochemistry , atpase , biophysics , biology , membrane , physics , materials science , nuclear physics , metallurgy , gene
In this paper the authors emphasise that the proton translocating ATP synthase from thiol‐modulated chloroplasts and two cyanobacterial strains has a coupling ratio of 4 protons per ATP synthesised or hydrolysed. This ratio is determined by several thermodynamic studies at equilibrium between phosphate potential ( ΔG p ) and proton gradient ( ), and is confirmed by measurement of proton flux during ATP hydrolysis. Ratios lower than 4 H + /ATP that have been published in the past have predominantly been determined with the oxidised chloroplast enzyme. Errors in these measurements will be discussed.