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Accumulation of nitrotyrosine on the SERCA2a isoform of SR Ca‐ATPase of rat skeletal muscle during aging: a peroxynitrite‐mediated process?
Author(s) -
Viner Rosa I.,
Ferrington Deborah A.,
Hühmer Andreas F.R.,
Bigelow Diana J.,
Schöneich Christian
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)01530-2
Subject(s) - peroxynitrite , nitrotyrosine , gene isoform , chemistry , skeletal muscle , biophysics , atpase , microbiology and biotechnology , biochemistry , biology , endocrinology , enzyme , nitric oxide synthase , superoxide , gene
The SR Ca‐ATPase in skeletal muscle SR vesicles isolated from young adult (5 months) and aged (28 months) rats was analyzed for nitrotyrosine. Only the SERCA2a isoform contained significant amounts with approximately one and four nitrotyrosine residues per young and old Ca‐ATPase, respectively. The in vitro exposure of SR vesicles of young rats to peroxynitrite yielded selective nitration of the SERCA2a Ca‐ATPase even in the presence of excess SERCA1a. No nitration was observed during the exposure of SR vesicles to nitric oxide in the presence of O 2 . These data suggest the in vivo presence of peroxynitrite in skeletal muscle. The greater nitrotyrosine content of SERCA2a from aged tissue imolies an age‐associated increase in susceptibility to oxidation by this species.