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Cellular processing of the neurotrophin precursors of NT3 and BDNF by the mammalian proprotein convertases
Author(s) -
Seidah Nabil G.,
Benjannet Suzanne,
Pareek Sangeeta,
Chrétien Michel,
Murphy Richard A.
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)01520-5
Subject(s) - furin , proprotein convertases , gene isoform , neurotrophin 3 , golgi apparatus , microbiology and biotechnology , neurotrophin , transfection , proprotein convertase , neurotrophic factors , chemistry , biochemistry , biology , brain derived neurotrophic factor , enzyme , receptor , gene , lipoprotein , ldl receptor , endoplasmic reticulum , cholesterol
In order to define the enzymes responsible for the maturation of the precursors of brain‐derived neurotrophic factor (proBDNF) and neurotrophin‐3 (proNT3), we have analysed their biosynthesis and intracellular processing by the proprotein convertases furin, PC1, PC2, PACE4, PC5 and its isoform PC5/6‐B. In these studies, we utilized a vaccinia virus expression system in either BSC40 or the furin activity‐deficient LoVo cells. Results demonstrated that in both cells furin and, to a lesser extent, PACE4 and PC5/6‐B effectively process proBDNF and proNT3. Furthermore, we have determined that human proNT3 is sulfated, suggesting that processing of proNT3 occurs following the arrival of the precursor to the Trans Golgi Network.