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Opposite regulation of bilirubin and 4‐nitrophenol UDP‐glucuronosyltransferase mRNA levels by 3,3′,5 triiodo‐ l ‐thyronine in rat liver
Author(s) -
Masmoudi Taoufik,
Planells Richard,
Mounié Jacques,
Artur Yves,
Magdalou Jacques,
Goudonnet Hervé
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)01507-8
Subject(s) - bilirubin , chemistry , glucuronosyltransferase , thyronine , nitrophenol , medicine , endocrinology , messenger rna , biochemistry , enzyme , biology , triiodothyronine , gene , hormone , microsome , catalysis
The effects of 3,3′,5 triiodo‐ l ‐thyronine (L‐T3) on the constitutive levels of hepatic mRNA encoding two UDP‐glucuronosyltransferase (UGT) isoforms implicated in the glucuronidation of planar phenolic substrates (UGTI ∗ 06) and bilirubin (UGT1 ∗ 0) were investigated in rat liver. The amount of UGT mRNA was quantitated by reverse transcription and amplification methods (RT‐PCR). Treatment with L‐T3 significantly increased UGT1 ∗ 06 and decreased UGT1 ∗ 0 mRNA levels by 41% and 54%, respectively. The opposite situation was observed in thyroidectomised animals. A good relationship observed between UGT activity toward 4‐nitrophenol and bilirubin and mRNA levels emphasizes the key role played by the thyroid hormone L‐T3 on UGT expression.