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Expression of human inducible nitric oxide synthase in Escherichia coli
Author(s) -
Fossetta James D.,
Niu Xiao Da,
Lunn Charles A.,
Zavodny Paul J.,
Narula Satwant K.,
Lundell Daniel
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)01496-9
Subject(s) - tetrahydrobiopterin , nitric oxide synthase , escherichia coli , recombinant dna , nitric oxide , atp synthase , chemistry , cofactor , calmodulin , biochemistry , microbiology and biotechnology , enzyme , biology , gene , organic chemistry
We have expressed active full‐length human inducible nitric oxide synthase (iNOS) in E. coli . Expression required co‐expression with calmodulin, a particularly tight‐binding cofactor. The extracts also required tetrahydrobiopterin to display activity. Specific activity of the purified recombinant iNOS was similar to iNOS purified from murine macrophages. This result indicates that no special processing events unique to eucaryotic cells are necessary for iNOS activity.