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Elevated cPLA 2 levels as a mechanism by which the p70 TNF and p75 NGF receptors enhance apoptosis
Author(s) -
MacEwan David J.
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)01495-0
Subject(s) - nerve growth factor , receptor , apoptosis , programmed cell death , tumor necrosis factor alpha , low affinity nerve growth factor receptor , microbiology and biotechnology , neurotrophin , medicine , endocrinology , trk receptor , cell , phospholipase c , chemistry , biology , biochemistry
The 70‐kDa TNF cell surface receptor (p70TNFR) and the related 75‐kDa nerve growth factor (NGF) receptor (p75NGFR) can enhance cell death. Expression of p70TNFR or p75NGFR in HeLa cells resulted in enhanced TNF‐induced apoptotic cell death with a corresponding elevation of cytosolic phospholipase A 2 (cPLA 2 ) levels. This response was apparent by 24 h, did not occur with NGF treatment or when vector or Trk A NGFR were expressed and was reversed by dexamethasone pretreatment. These findings reveal a novel mechanism by which the p70TNFR and p75NGFR achieve enhanced TNFR‐mediated programmed cell death by elevating cPLA 2 levels.