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3D solution structure of copper and silver‐substituted yeast metallothioneins
Author(s) -
Peterson Cynthia W.,
Narula Surinder S.,
Armitage Ian M.
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)01492-6
Subject(s) - dihedral angle , copper , chemistry , crystallography , metal , yeast , hydrogen bond , metallothionein , cluster (spacecraft) , stereochemistry , molecule , biochemistry , organic chemistry , zinc , computer science , programming language
3D solution structural calculations for yeast silver(I)‐substituted metallothionein (MT) and native copper(I) MT were completed using experimentally determined NOE and dihedral angle constraints, in conjunction with experimentally derived metal‐to‐Cys connectivities for AgMT which were assumed identical for CuMT. For the first 40 residues in both structures, the polypeptide backbone wraps around the metal cluster in two large parallel loops separated by a deep cleft containing the metal cluster. Minor differences between the two structures include differences in hydrogen bonds and the orientation of the N‐terminus with the overall protein volume conserved to within 6.5%.