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Cloning and expression of a bovine adenylyl cyclase type VII specific to the retinal pigment epithelium
Author(s) -
Völkel H.,
Beitz E.,
Klumpp S.,
Schultz J.E.
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)01470-5
Subject(s) - adenylyl cyclase , microbiology and biotechnology , retinal pigment epithelium , gene isoform , biology , complementary dna , adcy9 , messenger rna , forskolin , adcy10 , gs alpha subunit , gtp' , biochemistry , gene , retinal , signal transduction , receptor , enzyme
A cDNA of a type 7 adenylyl cyclase isoform was cloned from a bovine retinal pigment epithelium cDNA library using oligonucleotides developed to conserved regions common to mammalian adenylyl cyclases. A 6.7 kb mRNA of very high abundance was uniquely present on Northern blots containing mRNA or total RNA from the pigment epithelium. This transcript was undetectable in all other tissues examined. The cDNA encoded a protein of 1,097 amino acids and exhibited the known doublet of 6 transmembrane‐spanning regions in a hydrophobicity plot. The novel member of the type 7 adenylyl cyclase isoform was expressed in COS‐1 cells. It was stimulated 10‐ and 20‐fold by 10 μM GTPγS and 100 μM forskolin, respectively. The high expression rate exclusively in the retinal pigment epithelium suggests that this adenylyl cyclase isoform is involved in processes specific to this functionally exceedingly important subretinal cell layer.