Premium
The TRH‐like peptides in rabbit testis are different from the TRH‐like peptide in the prostate
Author(s) -
Linden Helena,
del Rio Garcia Jesus,
Huber Ariana,
Kreil Günther,
Smyth Derek
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)01468-3
Subject(s) - peptide , rabbit (cipher) , prostate , endocrinology , medicine , chemistry , biology , microbiology and biotechnology , biochemistry , computer science , cancer , computer security
Human seminal fluid contains a number of tripeptide amides with similar structures to thyrotropin releasing hormone (TRH), two of which have been identified as pGlu‐Glu‐Pro amide and pGlu‐Phe‐Pro amide. To determine whether these peptides originate in the same tissues and have the same molecular origin, TRH‐immunoreactive peptides were extracted from the prostate and testis of the rabbit, purified by ion exchange chromatography and HPLC, and identified by co‐chromatography with 3 H‐labelled marker peptides. In addition, trypsin digestion was used to release TRH‐like tripeptides from N‐extended forms of these peptides. The sole TRH‐like peptide in the prostate was shown to be pGlu‐Glu‐Pro amide; it was not accompanied by a detectable amount of pGlu‐Phe‐Pro amide. The prostate also appeared to contain a very small amount of N‐extended forms of these peptides. In contrast to the prostate, the testis contained high concentrations of N‐extended forms of pGlu‐Phe‐Pro amide but essentially no tripeptide. The testis also contained N‐extended forms of two other neutral TRH‐like peptides which were less hydrophobic than pGlu‐Phe‐Pro amide. Neither the prostate nor the testis contained a significant amount of TRH. The results show that in the rabbit the TRH‐like peptides pGlu‐Glu‐Pro amide and pGlu‐Phe‐Pro amide occur in different tissues and appear to be formed from different precursors.